A New Insight Into the Anti Proliferative and Apoptotic Effects of Fulvic and Humic Acids as Bio Product of Humus on Breast Cancer Cells, Optimized by Response Surface Methodology

Today, plant compounds and substances of natural origin as bio products are strongly recommended for the prevention treatment cancer. In field biomedicine anti-viral, anti-inflammatory, anti-oxidant properties among most known activities Humus obtained from humus. this study, effect humic fulvic acids (HA, FA), product humus, on breast cancer cells (MCF7), common women, was investigated. To achieve optimum cytotoxic time determine different parameters Response Surface Methodology (RSM) applied. The main influencing performance in MTT assay, such concentrations were regarded. cell viability measured using HA FA including 10, 50, 100, 200 µg/mL 14, 24, 48 h, respectively. Apoptosis, cycle, mechanical survivin gene expression MCF7 treated with analyzed after 14 h. Our results showed that induced apoptosis, reduced cured cells. We have seen a dose-dependent behavior increasing population phase Sub-G1. AFM elastic modulus value cell–cell adhesion forces FA. golden result study matching laboratory statistical which confirms success RSM model biological researches.